Acetyl-CoA Carboxylase beta antibody (pSer220)

Catalog No. ABIN1713595

This Rabbit Polyclonal antibody specifically detects Acetyl-CoA Carboxylase beta in ELISA, IF (cc), IF (p), ICC, IHC (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for Acetyl-CoA Carboxylase beta antibody (pSer220) (ABIN1713595)

Target: Acetyl-CoA Carboxylase beta (ACACB)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Acetyl-CoA Carboxylase beta antibody is un-conjugated

Application: ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Acetyl-CoA Carboxylase beta Antibody

Binding Specificity: pSer220

Predicted Reactivity: Human

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Acetyl Coenzyme A Carboxylase beta around the phosphorylation site of Ser220

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for Acetyl-CoA Carboxylase beta

Target: Acetyl-CoA Carboxylase beta (ACACB)

Alternative Name: Acetyl Coenzyme A Carboxylase beta

Background:

Synonyms: Acetyl Coenzyme A Carboxylase beta phospho S220, Acetyl Coenzyme A Carboxylase beta phospho Ser2210 p-Acetyl Coenzyme A Carboxylase beta phospho S220, ACACB_HUMAN

Background: Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008].

UniProt: O00763

Pathways: AMPK Signaling, Ribonucleoside Biosynthetic Process