FFAR1 antibody (AA 121-220)

Catalog No. ABIN1714627

This Rabbit Polyclonal antibody specifically detects FFAR1 in WB, ELISA, IF (cc), IF (p), IHC (p), ICC and IHC (fro). It exhibits reactivity toward Human and Mouse.

Quick Overview for FFAR1 antibody (AA 121-220) (ABIN1714627)

Target: FFAR1 (Free Fatty Acid Receptor 1 (FFAR1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FFAR1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-FFAR1 Antibody

Binding Specificity: AA 121-220

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Dog,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human GPR40

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for FFAR1

Target: FFAR1 (Free Fatty Acid Receptor 1 (FFAR1))

Alternative Name: GPR40

Background:

Synonyms: FFA1R, GPR40, GPCR40, Free fatty acid receptor 1, G-protein coupled receptor 40, FFAR1

Background: G-protein coupled receptor for medium and long chain saturated and unsaturated fatty acids that plays an important role in glucose homeostasis. Fatty acid binding increases glucose-stimulated insulin secretion, and may also enhance the secretion of glucagon-like peptide 1 (GLP-1). May also play a role in bone homeostasis, receptor signaling activates pathways that inhibit osteoclast differentiation (By similarity). Ligand binding leads to a conformation change that triggers signaling via G-proteins that activate phospholipase C, leading to an increase of the intracellular calcium concentration. Seems to act through a G(q) and G(i)-mediated pathway.

Gene ID: 2864

UniProt: O14842

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Peptide Hormone Metabolism, Carbohydrate Homeostasis