ENOS antibody (pSer615)

Catalog No. ABIN1714908

The Rabbit Polyclonal anti-ENOS antibody (ABIN1714908) specifically detects ENOS in WB, ELISA, ICC, IF (cc), IF (p), IHC (p) and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for ENOS antibody (pSer615) (ABIN1714908)

Target: ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ENOS antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ENOS Antibody

Binding Specificity: pSer615

Cross-Reactivity: Human

Predicted Reactivity: Dog,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human eNOS around the phosphorylation site of Ser615

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for ENOS

Target: ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))

Alternative Name: eNOS

Background:

Synonyms: eNOS phospho S615, p-eNOS phospho S615, eNOS Ser615, cNOS, eNOS, Constitutive NOS, EC NOS, ecNOS, Endothelial nitric oxidase synthase, Endothelial nitric oxide synthase, Endothelial nitric oxide synthase 3, Endothelial NOS, Nitric oxide synthase 3 endothelial cell, Nitric oxide synthase 3, Nitric oxide synthase 3 endothelial cell, Nitric oxide synthase endothelial, nitric oxide synthase, endothelial, NOS 3, NOS III, NOS type III, NOS3, NOSIII, NOS3_HUMAN.

Background: eNOS is a calcium/calmodulin dependent enzyme which undergoes several post translational modifications, including acylation with myristate and palmitate, and phosphorylation on numerous residues. As with the other members of the NOS family, eNOS derives the diffusible multifunctional second messenger NO from L arginine through a series of reactions in which L citrulline is an intermediate. eNOS plays an important role in controlling vascular tone, platelet aggregation, and cardiac myocyte function.

Gene ID: 4846

Pathways: ACE Inhibitor Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Response to Molecule of Bacterial Origin, Myometrial Relaxation and Contraction, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling