Hephaestin antibody (HEPH)

Details for Product anti-HEPH Antibody No. ABIN1715088, Supplier: Log in to see
Antigen
  • HEPH
  • C130006F04Rik
  • Cpl
  • mKIAA0698
  • sla
  • CPL
  • hephaestin
  • HEPH
  • heph
  • Heph
Alternatives
anti-Human Hephaestin antibody for ELISA
Reactivity
Human, Rat (Rattus)
37
26
10
2
1
1
1
1
1
1
Host
Rabbit
33
6
Clonality
Polyclonal
Conjugate
This Hephaestin antibody is un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
21
17
13
12
3
2
2
1
Options
Supplier
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Immunogen KLH conjugated synthetic peptide derived from human Hephaestin
Isotype IgG
Purification Purified by Protein A.
Plasmids, Primers & others Plasmids, Primers & others Hephaestin products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name Hephaestin (HEPH Antibody Abstract)
Background

Hephaestin is a single-pass type I membrane protein that belongs to the multicopper oxidase family of proteins. Hephaestin, a copper-dependant ferroxidase protein, is crucial for iron exiting intestinal enterocytes into the circulation. It mediates the movement of iron across the basolateral membrane in conjunction with ferroportin 1. This is an important link between iron and copper metabolism in mammalian systems, as copper deficiency leads to reduced hephaestin and reduced iron absorption resulting in anemia. Hephaestin can bind six copper ions per monomer and is regulated by the homeobox transcription factor CDX2. Increased levels of iron leads to an increase in CDX2 expression and thus Hephaestin. Hephaestin is primarily detected in the intestine, but is also expressed in colon, breast, bone trabecural cells and fibroblasts.

Subcellular location: Extracellular

Synonyms: CPL, HEPH, HEPH_HUMAN, Hephaestin.

Gene ID 9843
Pathways Transition Metal Ion Homeostasis
Application Notes WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months
Product cited in: Rychtarcikova, Lettlova, Tomkova, Korenkova, Langerova, Simonova, Zjablovskaja, Alberich-Jorda, Neuzil, Truksa: "Tumor-initiating cells of breast and prostate origin show alterations in the expression of genes related to iron metabolism." in: Oncotarget, Vol. 8, Issue 4, pp. 6376-6398, 2016 (PubMed). Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Human).

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