DNMT1 antibody (AA 637-650)

Catalog No. ABIN1721507

The Mouse Monoclonal anti-DNMT1 antibody is suitable to detect DNMT1 in samples from Human, Mouse and Zebrafish (Danio rerio). It has been validated for WB and ELISA.

Quick Overview for DNMT1 antibody (AA 637-650) (ABIN1721507)

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Reactivity: Human, Mouse, Zebrafish (Danio rerio)

Host: Mouse

Clonality: Monoclonal

Conjugate: This DNMT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 4G11-C7

Product Details anti-DNMT1 Antibody

Binding Specificity: AA 637-650

Specificity: Reacts with human, mouse and zebrafish DNMT1.

Purification: Purified (protein G)

Immunogen: Synthetic peptide corresponding to aa 637-650 of human DNMT1 (accession no. NP_001370).

Isotype: IgG2b

Application Details

Application Notes: Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
WB(2 μg/mL)

Positive control: ES and H1299 cell lysates.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS (2 mg/mL), pH 7.4.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute in PBS or medium which is identical to that used in the assay system.

Storage: -20 °C

Storage Comment: This antibody is stable for at least one year at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for DNMT1

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Alternative Name: DNMT1

Background: Methylation of DNA at cytosine residues is important in regulation of gene expression and genomic imprinting. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked to development of cancer. Thus far three families of mammalian DNA methyltransferase genes have been identified : DNMT1, DNMT2, and DNMT3. DNMT1 is constitutively expressed in proliferating cells, and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. DNMT1 co-purifies with the retinoblastoma (Rb) tumor suppressor gene product E2F1 and with HDAC1. DNMT1 cooperates with Rb to repress transcription from promoters containing E2F-binding sites which suggests a link between DNA methylation, histone deacetylase and sequence specific DNA binding activity as well as a growth-regulatory pathway that is disrupted in cancer cells.

DNA (cytosine-5)-methyltransferase 1 CXXC-type zinc finger protein 9, CXXC9 DNA methyltransferase HsaI, DNA MTase HsaI, M.HsaI Gene name : DNMT1

Gene ID: 1786

UniProt: P26358

Pathways: SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection