SYT1/2 antibody (Cytoplasmic)

Catalog No. ABIN1742196

Product Details anti-SYT1/2 Antibody

Target: SYT1/2 (Synaptotagmin 1/2 (SYT1/2))

Binding Specificity: AA 120-131, Cytoplasmic

Reactivity: Human, Mouse, Rat, Chicken, Cow, Goldfish

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SYT1/2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Cross-Reactivity (Details): Some cross-reactivity to synaptotagmin 2.

Purification: antiserum

Immunogen: Synthetic peptide DDDAETGLTDGE (aa 120-131 in rat synaptotagmin 1) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
ICC: 1 : 500 up to 1 : 1000
IHC: 1 : 200 up to 1 : 500

Comment:

ELISA: Suitable as detector antibody for sandwich-ELISA as capture antibodies (protocol for sandwich-ELISA).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS

Handling Advice: Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.

Storage: 4 °C/-20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-SYT1/2 antibody (ABIN1742196)

Rodriguez-Gil, Hu, Greer: "Dishevelled proteins are associated with olfactory sensory neuron presynaptic terminals." in: PLoS ONE, Vol. 8, Issue 2, pp. e56561, (2013) (PubMed).

Target Details for SYT1/2

Target: SYT1/2 (Synaptotagmin 1/2 (SYT1/2))

Alternative Name: Synaptotagmin 1, 2 (SYT1/2 Products)

Background: Synonyms: p65