Syntaxin 4 antibody (Cytoplasmic Domain)

Catalog No. ABIN1742222

Product Details anti-Syntaxin 4 Antibody

Target: Syntaxin 4 (STX4)

Binding Specificity: AA 1-273, Cytoplasmic Domain

Reactivity: Human, Mouse, Rat, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Syntaxin 4 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Specificity: Specific for syntaxin 4.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Recombinant cytoplasmic domain of rat syntaxin 4 (aa 1-273).

Application Details

Application Notes: WB: 1 : 1000 up to 1 : 5000 (AP staining)
ICC: 1 : 100
IHC: not tested yet

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

References for anti-Syntaxin 4 antibody (ABIN1742222)

Tao-Cheng, Pham, Yang, Winters, Gallant, Reese: "Syntaxin 4 is concentrated on plasma membrane of astrocytes." in: Neuroscience, Vol. 286, pp. 264-71, (2015) (PubMed).

Ulloa, Gonzàlez-Juncà, Meffre, Barrecheguren, Martínez-Mármol, Pazos, Olivé, Cotrufo, Seoane, Soriano: "Blockade of the SNARE protein syntaxin 1 inhibits glioblastoma tumor growth." in: PLoS ONE, Vol. 10, Issue 3, pp. e0119707, (2015) (PubMed).

Werno, Chamberlain: "S-acylation of the Insulin-Responsive Aminopeptidase (IRAP): Quantitative analysis and Identification of Modified Cysteines." in: Scientific reports, Vol. 5, pp. 12413, (2015) (PubMed).

Baron, Ozgen, Klunder, de Jonge, Nomden, Plat, Trifilieff, de Vries, Hoekstra: "The major myelin-resident protein PLP is transported to myelin membranes via a transcytotic mechanism: involvement of sulfatide." in: Molecular and cellular biology, Vol. 35, Issue 1, pp. 288-302, (2014) (PubMed).

Target Details for Syntaxin 4

Target: Syntaxin 4 (STX4)

Alternative Name: Syntaxin 4 (STX4 Products)

Synonyms: CG2715 antibody, Dm Syx4 antibody, DmSyx4 antibody, Dmel\\CG2715 antibody, EG:95B7.1 antibody, EG:95B7.11 antibody, Syx antibody, Syx-4 antibody, syx4 antibody, STX4 antibody, p35-2 antibody, stx4a antibody, syntaxin4 antibody, STX4A antibody, Stx4a antibody, Stx4 antibody, Syn-4 antibody, Syn4 antibody, Syntaxin 4 antibody, syntaxin 4 antibody, syntaxin 4 L homeolog antibody, syntaxin 4A (placental) antibody, Syx4 antibody, STX4 antibody, stx4 antibody, stx4.L antibody, Stx4 antibody, Stx4a antibody

Pathways: Synaptic Vesicle Exocytosis