GFP antibody (AA 183-191)

Catalog No. ABIN1742292

This anti-GFP antibody is a Mouse Monoclonal antibody detecting GFP in WB. Suitable for Aequorea victoria. This Primary Antibody has been cited in 3+ publications.

Quick Overview for GFP antibody (AA 183-191) (ABIN1742292)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Mouse

Clonality: Monoclonal

Conjugate: This GFP antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 101G4

Product Details anti-GFP Antibody

Binding Specificity: AA 183-191

Specificity: Specific for GFP of jelly-fish.

Immunogen: Recombinant full length GFP, glutaraldehyde fixed. Epitope QQNTPIGDG (aa 183-191)

Isotype: IgG2b

Application Details

Application Notes: WB: 1:1000 up to 1:10000, IP: not recommended IHC: not recommended

Comment:

ABIN1742292 is recommended for IP and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100µl H2O to get a 1mg/ml solution in PBS.

Buffer: PBS. Azide was added before lyophilization.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C

Storage Comment: Reconstitute and aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized.

References for anti-GFP antibody (ABIN1742292)

Koestler, Steffen, Nemethova, Winterhoff, Luo, Holleboom, Krupp, Jacob, Vinzenz, Schur, Schlüter, Gunning, Winkler, Schmeiser, Faix, Stradal, Small, Rottner: "Arp2/3 complex is essential for actin network treadmilling as well as for targeting of capping protein and cofilin." in: Molecular biology of the cell, Vol. 24, Issue 18, pp. 2861-75, (2013) (PubMed).

Oelkers, Vinzenz, Nemethova, Jacob, Lai, Block, Szczodrak, Kerkhoff, Backert, Schlüter, Stradal, Small, Koestler, Rottner: "Microtubules as platforms for assaying actin polymerization in vivo." in: PLoS ONE, Vol. 6, Issue 5, pp. e19931, (2011) (PubMed).

Kaddai, Gonzalez, Keslair, Grémeaux, Bonnafous, Gugenheim, Tran, Gual, Le Marchand-Brustel, Cormont: "Rab4b is a small GTPase involved in the control of the glucose transporter GLUT4 localization in adipocyte." in: PLoS ONE, Vol. 4, Issue 4, pp. e5257, (2009) (PubMed).

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP