GFP antibody (CypHer5E)

Catalog No. ABIN1742297

The Rabbit Polyclonal anti-GFP antibody has been validated for ICC. It is suitable to detect GFP in samples from Aequorea victoria. There is 1 publication available.

Quick Overview for GFP antibody (CypHer5E) (ABIN1742297)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFP antibody is conjugated to CypHer5E

Application: Immunocytochemistry (ICC)

Product Details anti-GFP Antibody (CypHer5E)

Cross-Reactivity (Details): Cross reacts with all GFP derivatives tested so far (e.g. EYFP, ECFP, EGFP).

Purification: Affinity purified with the immunogen, fluorescence-labeled with CypHer5E. Rabbit serum albumin was added for stabilization.

Immunogen: Strep-Tag® fusion protein, full-length GFP.

Application Details

Application Notes: IP: not tested yet
ICC: 1 : 100 up to 1 : 1000
IHC-P: not tested yet

Comment:

ABIN1742297 Suitable for internalization studies when GFP is fused to an epitope residing in the lumen of an acidic compartment.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Either add 1:1 (v/v) glycerol (if this does not interfere with your experiments!), then aliquot and store at -20 °C until use, or store aliquots at -80 °C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Fluorescence-labeled antibodies should be reconstituted immediately upon delivery.
Prolonged storage at 4 °C is not recommended!

References for anti-GFP antibody (CypHer5E) (ABIN1742297)

Tatavarty, Sun, Turrigiano: "How to scale down postsynaptic strength." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 32, pp. 13179-89, (2013) (PubMed).

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP