GFP antibody (CypHer5E)
(1 reference)Quick Overview for GFP antibody (CypHer5E) (ABIN1742297)
Target
See all GFP AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Purpose
- Rabbit anti-GFP antibody
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Characteristics
- fluorescence-labeled with Cy pH er5E. Cy pH er5E® is a pH -sensitive dye, fluorescent only after antibody internalization at the acidic pH of the lumen of the synaptic vesicle. Cy pH er5E® can be detected with the filter sets for Cy5®. Cy pH er5E®: λex 647 nm / λem 667 nm
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Purification
- affinity purified
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anti-Green Fluorescent Protein (GFP) antibody
GFP Reactivity: Aequorea victoria WB, ELISA, FM Host: Goat Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP Reactivity: Aequorea aequorea WB, IF, IHC (fro), IHC (p) Host: Goat Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP Reactivity: Aequorea victoria WB, ELISA, IP, IF, FACS Host: Mouse Monoclonal 6C11C11 unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP Reactivity: Aequorea victoria WB, ELISA, IP, IC, ChIP-seq Host: Rat Monoclonal 1A5 unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP Reactivity: Aequorea victoria WB, IHC, IP, ICC, FACS Host: Mouse Monoclonal 4B10B2 unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP Reactivity: Aequorea victoria WB, IF, IHC (fro), IHC (p), IEM Host: Goat Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP Reactivity: Aequorea victoria WB, ELISA, IHC, IP, FM, FACS, DB Host: Mouse Monoclonal 9F9-F9 unconjugated
anti-Green Fluorescent Protein (GFP) (AA 1-100) antibodyGFP Reactivity: Aequorea victoria WB, IF Host: Mouse Monoclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP Reactivity: Aequorea victoria WB, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibody (DyLight 550)GFP Reactivity: Aequorea victoria WB, IF, IHC (fro) Host: Goat Polyclonal DyLight 550
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Application Notes
- WB:N/A IP:N/A ICC:1 : 100 up to 1 : 1000, IHC:not tested yet IHC-P:not tested yet
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Comment
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ABIN1742297 Suitable for internalization studies when GFP is fused to an epitope residing in the lumen of an acidic compartment.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- For reconstitution add 50 μLH2O to get a 1 mg/mL solution in PBS.
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Buffer
- PBS
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Preservative
- Without preservative
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Handling Advice
- Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
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Storage
- -20 °C,-80 °C
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Storage Comment
- Albumin was added for stabilization. Reconstitute immediately upon receipt! Either add 1:1 (v/v) glycerol then aliquot and store at -20°C until use or store aliquots at -80°C without additives.
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: "How to scale down postsynaptic strength." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 32, pp. 13179-89, (2013) (PubMed).
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: "How to scale down postsynaptic strength." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 32, pp. 13179-89, (2013) (PubMed).
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- GFP (Green Fluorescent Protein (GFP))
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Alternative Name
- GFP
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Background
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Synonyms: Sirius, meGFP, eGFP, paGFP, acGFP, eYFP, sfGFP, Green fluorescent protein, supereclyptic pH luorin, Cerulean, eCFP, mTFP, Venus, Citrine
Antigen Information: GFP is a widely used green fluorescent protein tag
Target
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