GFP antibody

Catalog No. ABIN1742299

This Guinea Pig Polyclonal antibody specifically detects GFP in WB and ICC. It exhibits reactivity toward Aequorea victoria.

Quick Overview for GFP antibody (ABIN1742299)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This GFP antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Product Details anti-GFP Antibody

Cross-Reactivity (Details): Cross reacts with all GFP derivatives tested so far (e.g. EYFP, ECFP, EGFP).

Purification: Affinity purified with the immunogen. Guinea pig serum albumin was added for stabilization.

Immunogen: Strep-Tag® fusion protein, full-length GFP.

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 100 up to 1 : 500
IHC: not tested yet

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP