GRIA2 antibody (AA 278-292)

Catalog No. ABIN1742378

The Mouse Monoclonal anti-GRIA2 antibody has been validated for WB and IP. It is suitable to detect GRIA2 in samples from Rat. There is 1 publication available.

Quick Overview for GRIA2 antibody (AA 278-292) (ABIN1742378)

Target: GRIA2 (Glutamate Receptor, Ionotropic, AMPA 2 (GRIA2))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This GRIA2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: 1404A10

Product Details anti-GRIA2 Antibody

Binding Specificity: AA 278-292

Specificity: Specific for GluA 2.

Purification: purified IgG. Azide was added before lyophilization.

Immunogen: Synthetic peptide (aa 278-292 in rat GluA 2, unprocessed precursor) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue.

Isotype: IgG2b

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
ICC: not tested yet
IHC: not tested yet

Comment:

IP:For most effective IP use the solubilization protocol described in this ELISA protocol. Consider that protein-protein interaction may be affected.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS, 0.02% sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Storage: -20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-GRIA2 antibody (ABIN1742378)

Jedlicka, Vnencak, Krueger, Jungenitz, Brose, Schwarzacher: "Neuroligin-1 regulates excitatory synaptic transmission, LTP and EPSP-spike coupling in the dentate gyrus in vivo." in: Brain structure & function, Vol. 220, Issue 1, pp. 47-58, (2015) (PubMed).

Target Details for GRIA2

Target: GRIA2 (Glutamate Receptor, Ionotropic, AMPA 2 (GRIA2))

Alternative Name: GluA 2

Background: Synonyms: AMPA 2, GluR 2

Pathways: PI3K-Akt Signaling