WB: 1 : 1000 (AP staining) ICC: not tested yet IHC: not tested yet
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer
PBS
Handling Advice
Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
Storage
-20 °C
Storage Comment
Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Luchkina, Huupponen, Clarke, Coleman, Keinänen, Taira, Lauri: "Developmental switch in the kinase dependency of long-term potentiation depends on expression of GluA4 subunit-containing AMPA receptors." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, Issue 11, pp. 4321-6, (2014) (PubMed).