GAD65 antibody (N-Term)

Catalog No. ABIN1742417

The Mouse Monoclonal anti-GAD65 antibody has been validated for WB, IHC, ELISA, IP, ICC and IHC (p). It is suitable to detect GAD65 in samples from Human, Rat and Mouse. There are 3+ publications available.

Quick Overview for GAD65 antibody (N-Term) (ABIN1742417)

Target: GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This GAD65 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 26H1D1

Product Details anti-GAD65 Antibody

Binding Specificity: N-Term

Purpose: Mouse anti-GAD2 IgG

Specificity: Specific for GAD 2 / GAD 65

Cross-Reactivity (Details): Reacts with: rat (Q05683), mouse (P48320), human (Q05329). Other species not tested yet.

Purification: purified IgG

Immunogen: Recombinant protein corresponding to the amino terminus of mouse GAD2 (UniProt Id: P48320) Epitop AA 3 to 17 from mouse GAD2 (UniProt Id: P48320)

Isotype: IgG3

Application Details

Application Notes: WB:1 : 1000 , IP:yes ICC:1 : 500, IHC:1 : 200 up to 1 : 500, IHC-P:1 : 100 up to 1 : 1000, ELISA:yes

Comment:

WB: This antibody is less sensitive than the rabbit antibody. ELISA: Suitable as capture antibody for sandwich-ELISA with ABIN1742419 as detector antibody (protocol for sandwich-ELISA).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μLH2O to get a 1 mg/mL solution in PBS.

Buffer: PBS. Albumin and azide were added for stabilization.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C,-80 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Albumin and azide were added for stabilization. Do not freeze!

References for anti-GAD65 antibody (ABIN1742417)

Pan, Marrs, Ryan: "Vesicular glutamate transporter 1 orchestrates recruitment of other synaptic vesicle cargo proteins during synaptic vesicle recycling." in: The Journal of biological chemistry, Vol. 290, Issue 37, pp. 22593-601, (2015) (PubMed).

Liu, Bickford, Held, Nyitrai, Südhof, Kaeser: "The active zone protein family ELKS supports Ca2+ influx at nerve terminals of inhibitory hippocampal neurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 34, Issue 37, pp. 12289-303, (2014) (PubMed).

Coley, Ruffin, Moss, Hopfer, Boron: "Immunocytochemical identification of electroneutral Na⁺-coupled HCO₃⁻ transporters in freshly dissociated mouse medullary raphé neurons." in: Neuroscience, Vol. 246, pp. 451-67, (2013) (PubMed).

Target Details for GAD65

Target: GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))

Alternative Name: GAD2

Background:

Synonyms: Glutamic acid decarboxylase, GAD65, Glutamic acid decarboxylase 2, Glutamic acid decarboxylase 65

Antigen Information: GAD1 and 2 are involved in the synthesis of GABA, the major inhibitory neurotransmitter

UniProt: P48320