GAD65 antibody (N-Term)

Catalog No. ABIN1742419

This Rabbit Polyclonal antibody specifically detects GAD65 in WB, IHC, ELISA, IP, ICC and IHC (p). It exhibits reactivity toward Rat and Mouse and has been mentioned in 2+ publications.

Quick Overview for GAD65 antibody (N-Term) (ABIN1742419)

Target: GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GAD65 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GAD65 Antibody

Binding Specificity: N-Term

Purpose: Rabbit anti-GAD2 antibody

Specificity: Specific for GAD 2 / GAD 65.

Cross-Reactivity (Details): Reacts with: rat (P18088), mouse (P48318). Other species not tested yet.

Purification: affinity purified

Immunogen: Recombinant protein corresponding to the amino terminus of mouse GAD2 (UniProt Id: P48320)

Application Details

Application Notes: WB:1 : 1000 , IP:yes ICC:1 : 2000, IHC:1 : 1000, IHC-P:1 : 1000, ELISA:yes

Comment:

ICC/IHC: The crude antiserum is recommended. ELISA: Suitable as detector antibody for sandwich-ELISA with ABIN1742417 as capture antibody (protocol for sandwich-ELISA).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μLH2O to get a 1 mg/mL solution in PBS.

Buffer: PBS. Albumin and azide were added for stabilization.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C,-80 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Albumin and azide were added for stabilization. Do not freeze!

References for anti-GAD65 antibody (ABIN1742419)

Du, Chazalon, Bestaven, Leste-Lasserre, Baufreton, Cazalets, Cho, Garret: "Early GABAergic transmission defects in the external globus pallidus and rest/activity rhythm alteration in a mouse model of Huntington's disease." in: Neuroscience, Vol. 329, pp. 363-79, (2016) (PubMed).

Saliba, Gu, Yan, Moss: "Blocking L-type voltage-gated Ca2+ channels with dihydropyridines reduces gamma-aminobutyric acid type A receptor expression and synaptic inhibition." in: The Journal of biological chemistry, Vol. 284, Issue 47, pp. 32544-50, (2009) (PubMed).

Target Details for GAD65

Target: GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))

Alternative Name: GAD2

Background:

Synonyms: Glutamic acid decarboxylase, GAD65, Glutamic acid decarboxylase 2, Glutamic acid decarboxylase 65

Antigen Information: GAD1 and 2 are involved in the synthesis of GABA, the major inhibitory neurotransmitter

UniProt: P48320