ZYX antibody (AA 1-572)

Catalog No. ABIN1742558

The Mouse Monoclonal anti-ZYX antibody has been validated for WB, IP, ICC and IHC (p). It is suitable to detect ZYX in samples from Human, Mouse, Rat and Hamster. There are 11+ publications available.

Quick Overview for ZYX antibody (AA 1-572) (ABIN1742558)

Target: ZYX (Zyxin (ZYX))

Reactivity: Human, Mouse, Rat, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This ZYX antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 164D4

Product Details anti-ZYX Antibody

Binding Specificity: AA 1-572

Purpose: Mouse anti-Zyxin IgG

Specificity: Specific for zyxin.

Cross-Reactivity (Details): Reacts with: human (Q15942), rat, mouse (Q62523), hamster. Other species not tested yet.

Purification: purified IgG

Immunogen: Recombinant protein corresponding to AA 1 to 572 from human Zyxin (UniProt Id: Q15942) Epitop AA 352 to 357 from human Zyxin (UniProt Id: Q15942)

Isotype: IgG1

Application Details

Application Notes: WB:1 : 100 up to 1 : 2000 , IP:yes ICC:1 : 100, IHC:not tested yet IHC-P:1 : 200,

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μLH2O to get a 1 mg/mL solution in PBS.

Buffer: PBS. Albumin and azide were added for stabilization.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C,-80 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Albumin and azide were added for stabilization. Do not freeze!

References for anti-ZYX antibody (ABIN1742558)

Missirlis, Haraszti, Scheele, Wiegand, Diaz, Neubauer, Rechenmacher, Kessler, Spatz: "Substrate engagement of integrins α5β1 and αvβ3 is necessary, but not sufficient, for high directional persistence in migration on fibronectin." in: Scientific reports, Vol. 6, pp. 23258, (2016) (PubMed).

Gadhari, Charnley, Marelli, Brugger, Chiquet: "Cell shape-dependent early responses of fibroblasts to cyclic strain." in: Biochimica et biophysica acta, Vol. 1833, Issue 12, pp. 3415-25, (2013) (PubMed).

Delaroche, Cantrelle, Subra, Van Heijenoort, Guittet, Jiao, Blanchoin, Chassaing, Lavielle, Auclair, Sagan: "Cell-penetrating peptides with intracellular actin-remodeling activity in malignant fibroblasts." in: The Journal of biological chemistry, Vol. 285, Issue 10, pp. 7712-21, (2010) (PubMed).

Low, Mukhina, Srinivas, Ng, Murata-Hori: "Domain analysis of alpha-actinin reveals new aspects of its association with F-actin during cytokinesis." in: Experimental cell research, Vol. 316, Issue 12, pp. 1925-34, (2010) (PubMed).

Mori, Nakagami, Koibuchi, Miura, Takami, Koriyama, Hayashi, Sabe, Mochizuki, Morishita, Kaneda: "Zyxin mediates actin fiber reorganization in epithelial-mesenchymal transition and contributes to endocardial morphogenesis." in: Molecular biology of the cell, Vol. 20, Issue 13, pp. 3115-24, (2009) (PubMed).

Vollenbröker, George, Wolfgart, Saleem, Pavenstädt, Weide: "mTOR regulates expression of slit diaphragm proteins and cytoskeleton structure in podocytes." in: American journal of physiology. Renal physiology, Vol. 296, Issue 2, pp. F418-26, (2009) (PubMed).

Vervenne, Crombez, Delvaux, Janssens, Van de Ven, Petit: "Targeted disruption of the mouse Lipoma Preferred Partner gene." in: Biochemical and biophysical research communications, Vol. 379, Issue 2, pp. 368-73, (2009) (PubMed).

Guo, Wang: "Retrograde fluxes of focal adhesion proteins in response to cell migration and mechanical signals." in: Molecular biology of the cell, Vol. 18, Issue 11, pp. 4519-27, (2007) (PubMed).

Coutts, MacKenzie, Griffith, Black: "TES is a novel focal adhesion protein with a role in cell spreading." in: Journal of cell science, Vol. 116, Issue Pt 5, pp. 897-906, (2003) (PubMed).

Garvalov, Higgins, Sutherland, Zettl, Scaplehorn, Köcher, Piddini, Griffiths, Way: "The conformational state of Tes regulates its zyxin-dependent recruitment to focal adhesions." in: The Journal of cell biology, Vol. 161, Issue 1, pp. 33-9, (2003) (PubMed).

Rottner, Krause, Gimona, Small, Wehland: "Zyxin is not colocalized with vasodilator-stimulated phosphoprotein (VASP) at lamellipodial tips and exhibits different dynamics to vinculin, paxillin, and VASP in focal adhesions." in: Molecular biology of the cell, Vol. 12, Issue 10, pp. 3103-13, (2001) (PubMed).

Target Details for ZYX

Target: ZYX (Zyxin (ZYX))

Alternative Name: Zyxin

Background: Zyxin is involved in the regulation of actin dynamics

UniProt: Q15942