ATP5A1 antibody (Internal Region)

Catalog No. ABIN1782159

Product Details anti-ATP5A1 Antibody

Target: ATP5A1 (ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, alpha Subunit 1, Cardiac Muscle (ATP5A1))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This ATP5A1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: ATP5A1

Sequence: RVHGLRNVQA EE

Specificity: This antibody is expected to recognize all reported isoforms (NP_004037.1, NP_001244263.1, NP_001001935.1). Reported variants represent identical protein: NP_001244264.1, NP_001001935.1. Reported variants represent identical protein: NP_004037.1, NP_00100

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 55 kDa band observed in Human, Mouse, Rat and Pig Heart lysates (calculated MW of 54.5 kDa according to NP_001001935.1). Recommended concentration: 0.01-0.03 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ATP5A1

Target: ATP5A1 (ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, alpha Subunit 1, Cardiac Muscle (ATP5A1))

Alternative Name: ATP5A1 (ATP5A1 Products)

Synonyms: atp5a antibody, MGC108350 antibody, AI035633 antibody, AL022851 antibody, AL023067 antibody, Atpm antibody, D18Ertd206e antibody, Mom2 antibody, ATP5A antibody, ATP5AL2 antibody, ATPM antibody, MC5DN4 antibody, MOM2 antibody, OMR antibody, ORM antibody, hATP1 antibody, zgc:154103 antibody, ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle L homeolog antibody, ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle antibody, ATP synthase subunit alpha, mitochondrial antibody, ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1 antibody, atp5a1.L antibody, ATP5A1 antibody, atp5a1 antibody, LOC100395383 antibody, Atp5a1 antibody

Background: ATP5A1, ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle, ATP5A, ATP5AL2, ATPM, MOM2, OMR, ORM, hATP1, ATP synthase alpha chain, mitochondrial, ATP synthase subunit alpha, mitochondrial, ATP synthase, H+ transportin

Molecular Weight: Expected molecular weight 52 kDa

Gene ID: 498, 11946, 65262

NCBI Accession: NP_004037, NP_001244263, NP_001001935

Pathways: Proton Transport, Ribonucleoside Biosynthetic Process