TNF alpha antibody

Catalog No. ABIN181044

This anti-TNF alpha antibody is a Rat Monoclonal antibody detecting TNF alpha in FACS and EIA. Suitable for Human.

Quick Overview for TNF alpha antibody (ABIN181044)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: Flow Cytometry (FACS), Enzyme Immunoassay (EIA)

Clone: GMO1-17A2

Product Details anti-TNF alpha Antibody

Characteristics: Synonyms: TNF, TNF-a, TNFA, TNFSF2, Cachectin, Tumor necrosis factor ligand superfamily member 2

Purification: Affinity Chromatography on Protein G.

Isotype: IgG1

Application Details

Application Notes: ELISA: 1-5 μg/mL. Flow Cytometry: Use 10 μL of 1/10-1/50 diluted antibody to label 10^6 cells in 100 μL(Membrane permeabilisation is required).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: Tumor Necrosis Factor (TNF-Alpha)

Background: Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumour Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex. TNF alpha is predominantly expressed by macrophages, monocytes, neutrophils, T cells and NK cells stimulated by bacterial lipopolysaccharides.Synonyms: Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2

UniProt: P01375

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome