FGF4 antibody (Biotin)

Catalog No. ABIN181589

The Rabbit Polyclonal anti-FGF4 antibody has been validated for WB and EIA. It is suitable to detect FGF4 in samples from Human.

Quick Overview for FGF4 antibody (Biotin) (ABIN181589)

Target: FGF4 (Fibroblast Growth Factor 4 (FGF4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FGF4 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-FGF4 Antibody (Biotin)

Specificity: This antibody detects FGF-4.

Purification: Affinity chromatography

Immunogen: Highly pure (> 98 %) recombinant human FGF-4

Application Details

Application Notes: ELISA: Direct: To detect FGF-4 by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant FGF-4. Western Blot: To detect FGF-4 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant FGF-4 is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Expiry Date: 6 months

Target Details for FGF4

Target: FGF4 (Fibroblast Growth Factor 4 (FGF4))

Alternative Name: FGF4

Background: FGF4 is a heparin binding growth factor that is a member of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF4 signals through the FGFR 1c, 2c, 3c, and 4.Synonyms: Fibroblast growth factor 4, HST, HST-1, HSTF1, Heparin secretory-transforming protein, Heparin-binding growth factor 4, KS3, Transforming protein KS3

Gene ID: 2249

NCBI Accession: NP_001998

UniProt: P08620

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance