RELM alpha antibody (Biotin)

Catalog No. ABIN181629

The Biotin-conjugated Rabbit Polyclonal anti-RELM alpha antibody (ABIN181629) specifically detects RELM alpha in WB and EIA. The antibody is reactive with Mouse samples.

Quick Overview for RELM alpha antibody (Biotin) (ABIN181629)

Target: RELM alpha

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RELM alpha antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-RELM alpha Antibody (Biotin)

Specificity: This antibody detects RELM alpha.

Purification: Affinity chromatography

Immunogen: Highly pure (> 98 %) recombinant murine RELMalpha

Application Details

Application Notes: ELISA: Direct: To detect mRELMα by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL of this antibody is required. In conjunction withcompatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant mRELMα. Sandwich: To detect mRELMα by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL of this antibody is required. In conjunction withPolyclonal Anti-Murine RELMα as a capture antibody, it allows the detection of at least 0.2 -0.4 ng/well of recombinant mRELMα. Western Blot: To detect mRELMα by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant mRELMα is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Expiry Date: 6 months

Target Details for RELM alpha

Target: RELM alpha

Background: Synonyms: Cysteine-rich secreted protein A12-gamma, Cysteine-rich secreted protein FIZZ1, FIZZ1, PMNG1, Parasite-induced macrophage novel gene 1 protein, RELM-alpha, RELMalpha, RETNLA, Resistin-like alpha

Gene ID: 57262

NCBI Accession: NP_065255

UniProt: Q9EP95