ITGA4 antibody (FITC)

Catalog No. ABIN181733

The Mouse Monoclonal anti-ITGA4 antibody is suitable to detect ITGA4 in samples from Human. It has been validated for FACS.

Quick Overview for ITGA4 antibody (FITC) (ABIN181733)

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGA4 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: BU49

Product Details anti-ITGA4 Antibody (FITC)

Specificity: This antibody recognizes CD49d, also known as integrin alpha 4.

Purification: Affinity Chromatography on Protein G

Immunogen: Hyatt myeloma cell line. Remarks: Cells from immunised mice were fused with cells of the NSO myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: Neat - 1/10, use 10 μL of the suggested working dilution to label 1x10^6cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as presrevative and 1 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ITGA4

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Alternative Name: CD49d / ITGA4

Background: CD49d is a 150kD transmembrane glycoprotein, which may be cleaved to form 70kD and 80kD fragments. CD49d is expressed on a broad range of cells including lymphocytes, monocytes, eosinophils and thymocytes. The CD49d molecule forms heterodimers with either integrin Beta 1 (to form VLA-4) or beta 7 (to form alpha4 beta7), which mediate cell adhesion to VCAM-1and fibronectin.Synonyms: CD49 antigen-like family member D, Integrin alpha-4, Integrin alpha-IV, VLA-4, VLA4

Gene ID: 3676

NCBI Accession: NP_000876

UniProt: P13612

Pathways: Integrin Complex