CD11b antibody

Catalog No. ABIN182004

The Mouse Monoclonal anti-CD11b antibody (Clone OX-42) (ABIN182004) specifically detects CD11b in FACS, IP and IHC (fro). The antibody is reactive with Rat samples.

Quick Overview for CD11b antibody (ABIN182004)

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD11b antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: OX-42

Product Details anti-CD11b Antibody

Purification: Affinity chromatography on Protein G

Endotoxin Level: Low Endotoxin

Immunogen: Rat peritoneal macrophages. Spleen cells from immunised BALB/c mice were fused with cells of the NSO/U mouse myeloma cell line.

Isotype: IgG2a

Application Details

Application Notes: Immunoprecipitation. Immunohistochemistry on frozen sections: 1/50 - 1/100, Clone MRC OX-42 has been reported as being suitable for use on paraffin-embedded material following PLP fixation (periodate- lysine-paraformaldehyde). Flow Cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells or 100 μL whole blood.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.01 EU/ug

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2

Handling Advice: This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Store the antibody at 2 - 8 °C. DO NOT FREEZE!

Target Details for CD11b

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Alternative Name: CD11b

Background: The antigen is expressed on most macrophages, including resident and activated peritoneal macrophages and Kupffer cells and around 35 % of alveolar macrophages. The antibody also labels dendritic cells, granulocytes and microglial cells in the brain.Synonyms: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor

Gene ID: 10116

UniProt: Q63001

Pathways: Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation