Actin antibody (AA 3-12)

Catalog No. ABIN1825634

The Mouse Monoclonal anti-Actin antibody has been validated for WB, ELISA, IHC, IHC (p) and IHC (fro). It is suitable to detect Actin in samples from Human, Mouse, Rat, Rabbit, Pig, Chicken, Cow, Guinea Pig, Horse, Goat and Monkey.

Quick Overview for Actin antibody (AA 3-12) (ABIN1825634)

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Reactivity: Human, Mouse, Rat, Rabbit, Pig, Chicken, Cow, Guinea Pig, Horse, Goat, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 3B3

Product Details anti-Actin Antibody

Binding Specificity: AA 3-12

Specificity: Human Alpha Actin, Smooth Muscle

Immunogen: A peptide comprising the N-terminal nonapeptide of alpha-skeletal actin with an acetylated N-terminus (Ac-DEDETTALVC-COOH) coupled to keyhole limpet hemocyanin through the cysteine residue as. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Horse, Rabbit, Pig, Opossum, Guinea pig, Chicken, Platypus, Lizard (100%), Xenopus, Salmon, Medaka, Pufferfish, Zebrafish (90%).

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, IHC, IHC-Fr, IHC-P, WB (1:1000 - 1:5000)

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for Actin

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Alternative Name: ACTA1 / ASMA

Background: Name/Gene ID: ACTA1

Synonyms: ACTA1, ACTA, Actin, alpha skeletal muscle, Alpha-actin-1, CFTD, CFTD1, CFTDM, MPFD, NEM1, NEM2, ASMA, NEM3

Gene ID: 58

UniProt: P68133

Pathways: Caspase Cascade in Apoptosis, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development