HSV Tag antibody
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- Target
- HSV Tag
- Reactivity
- Herpes Simplex Virus (HSV)
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- Un-conjugated
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Application
- Western Blotting (WB), ELISA
- Sequence
- QPELAPEDPE DC
- Specificity
- This Antibody recognizes HSV tagged fusion proteins.
- Characteristics
- Rabbit Anti-HSV-tag Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography.
- Purification
- Immunoaffinity chromatography
- Immunogen
- HSV epitope tag peptide QPELAPEDPED conjugated to KLH
- Isotype
- IgG
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- Application Notes
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Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0. 05-0. 2 µg/mL
Western blot: 0. 1-1. 0 µg/mL
Other applications: user-optimized - Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final antibody concentration of 0.5 mg/mL.
- Concentration
- 0.5 mg/mL
- Buffer
- lyophilized with PBS, pH 7.4, containing 0.02 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- The antibody is stable in lyophilized form if stored at -20 °C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8 °C. For long term storage, aliquot and store at -20 °C or below.
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A Novel Peptide-Based SILAC Method to Identify the Posttranslational Modifications Provides Evidence for Unconventional Ubiquitination in the ER-Associated Degradation Pathway." in: International journal of proteomics, Vol. 2013, pp. 857918, (2013) (PubMed).
: "A functional study of nucleocytoplasmic transport signals of the EhNCABP166 protein from Entamoeba histolytica." in: Parasitology, Vol. 139, Issue 13, pp. 1697-710, (2012) (PubMed).
: "
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A Novel Peptide-Based SILAC Method to Identify the Posttranslational Modifications Provides Evidence for Unconventional Ubiquitination in the ER-Associated Degradation Pathway." in: International journal of proteomics, Vol. 2013, pp. 857918, (2013) (PubMed).
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- Target
- HSV Tag
- Target Type
- Tag
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