(2 references)-
- Target
- TF Tag
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- ELISA, Western Blotting (WB)
- Specificity
- Rabbit Anti-TF-tag polyclonal antibody specifically reacts with fusion proteins containing TF tag. The antibody can also detect endogenous levels of E.coli trigger factor.
- Characteristics
- Rabbit Anti-TF-tag polyclonal Antibody is developed in rabbit using purified recombinant TF protein. This polyclonal antibody is highly purified from rabbit antiserum by immunoaffinity chromatography.
- Purification
- Immunoaffinity chromatography
- Immunogen
- Purified recombinant TF protein
- Isotype
- IgG
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- Application Notes
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Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0. 05-0. 2 µg/mL
Western blot: 0. 2-1 µg/mL
Immunoprecipitation (IP): 2-10 µg/mg of lysate - Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final antibody concentration of 0.5 mg/mL.
- Concentration
- 0.5 mg/mL
- Buffer
- lyophilized with PBS, pH 7.4, containing 0.02 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
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Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap.
Avoid repeated freezing and thawing. - Storage
- -20 °C
- Storage Comment
- The antibody is stable in lyophilized form if stored at -20 °C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8 °C. For long term storage, aliquot and store at -20 °C or below.
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: "Cotranslational protein folding within the ribosome tunnel influences trigger-factor recruitment." in: Biophysical journal, Vol. 102, Issue 12, pp. 2818-27, (2012) (PubMed).
: "Bacterial protein translocation requires only one copy of the SecY complex in vivo." in: The Journal of cell biology, Vol. 198, Issue 5, pp. 881-93, (2012) (PubMed).
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: "Cotranslational protein folding within the ribosome tunnel influences trigger-factor recruitment." in: Biophysical journal, Vol. 102, Issue 12, pp. 2818-27, (2012) (PubMed).
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- Target
- TF Tag
- Target Type
- Tag
- Background
- Well-characterized antibodies for epitope tags have been widely used in the study of protein expression in various systems.Trigger Factor (TF) is a prokaryotic ribosome-associated chaperone protein (48 kDa) which facilitates co-translational folding of newly expressed proteins, thus enabling efficient soluble protein production for otherwise intractable target proteins
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