GFP antibody

Catalog No. ABIN1843725

This anti-GFP antibody is a Mouse Monoclonal antibody detecting GFP in WB, ELISA, IHC and FACS. Suitable for Aequorea victoria.

Quick Overview for GFP antibody (ABIN1843725)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Mouse

Clonality: Monoclonal

Conjugate: This GFP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-GFP Antibody

Purification: Purified antibody

Immunogen: Purified recombinant fragment of GFP expressed in E. coli.

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles.

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP

Background: GFP (Green fluorescence protein) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light when excited by blue light.GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors.GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells.GFP fluorescence is stable under fixation conditions and suitable for a variety of applications.GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols.GFP technnology has considerably contributed to a greater understanding of cellular physiology.

Molecular Weight: 27 kDa