Aurora A antibody (C-Term)

Catalog No. ABIN184572

This anti-Aurora A antibody is a Goat Polyclonal antibody detecting Aurora A in WB, ELISA and IP. Suitable for Human.

Quick Overview for Aurora A antibody (C-Term) (ABIN184572)

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Aurora A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Grade: Verified

Product Details anti-Aurora A Antibody

Binding Specificity: C-Term

Purpose: Aurora kinase A / AURKA

Sequence: QNKESASKQS

Specificity: Reported variants represent identical protein: NP_003591.2, NP_940835.1, NP_940836.1, NP_940837.1, NP_940838.1, NP_940839.1

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QNKESASKQS, from the C Terminus of the protein sequence according to NP_003591.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 45 kDa band observed in lysates of cell line HeLa and Jurkat lysate (calculated MW of 45.8 kDa according to NP_003591.2). Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunoprecipitation: This antibody was deemed fit for IP under native conditions (observations from a customer).

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Aurora A

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Alternative Name: AURKA

Background: AURKA, aurora kinase A, AIK, ARK1, AURA, AURORA2, BTAK, MGC34538, STK15, STK6, STK7, IPL1-related kinase, aurora-A, aurora-related kinase 1, aurora/IPL1-like kinase, breast-tumor-amplified kinase, serine/threonine kinase 15, serine/threonine kinase 6, ser

Gene ID: 6790

NCBI Accession: NP_003591

Pathways: Cell Division Cycle, Asymmetric Protein Localization