CDKN1B antibody (C-Term)

Catalog No. ABIN184587

The Goat Polyclonal anti-CDKN1B antibody (ABIN184587) specifically detects CDKN1B in WB, ELISA and IP. The antibody is reactive with Human samples.

Quick Overview for CDKN1B antibody (C-Term) (ABIN184587)

Target: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CDKN1B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Grade: Verified

Product Details anti-CDKN1B Antibody

Binding Specificity: C-Term

Purpose: P27KIP1 / CDKN1B (C Terminus)

Sequence: EQTPKKPGLR RRQT

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EQTPKKPGLRRRQT, from the C Terminus of the protein sequence according to NP_004055.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 26-27 kDa band observed in Human Breast and Prostate lysates (calculated MW of 22.1 kDa according to NP_004055.1). The observed molecular weight corresponds to earlier findings in literature (Xia et al, Proc Natl Acad Sci U S A. 2005 S

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunoprecipitation:This antibody was deemed fit for IP under native conditions (observations from anonymous customer).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CDKN1B

Target: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Alternative Name: CDKN1B

Background: CDKN1B, P27KIP1, cyclin-dependent kinase inhibitor 1B (p27, Kip1), KIP1, CDKN4, cyclin-dependent kinase inhibitor 1B, p27(KIP1), KIP1, p27, MEN1B, MEN4, P27KIP1

Gene ID: 1027, 12576, 83571

NCBI Accession: NP_004055

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy