ATF2 antibody (C-Term)

Catalog No. ABIN184639

This Goat Polyclonal antibody specifically detects ATF2 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for ATF2 antibody (C-Term) (ABIN184639)

Target: ATF2 (Activating Transcription Factor 2 (ATF2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ATF2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-ATF2 Antibody

Binding Specificity: C-Term

Purpose: ATF2

Sequence: IVMAPSSQSQ PSGS

Cross-Reactivity: Cow, Dog, Human, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-IVMAPSSQSQPSGS, from the C Terminus of the protein sequence according to NP_001871.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 75 kDa observed in nuclear lysates of cell lines HeLa, HepG2 and K562. This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ATF2

Target: ATF2 (Activating Transcription Factor 2 (ATF2))

Alternative Name: ATF2

Background: ATF2, activating transcription factor 2, HB16, CREB2, TREB7, CRE-BP1, cAMP responsive element binding protein 2, MGC111558, activating transcription factor 2 splice variant ATF2-var2, cAMP responsive element binding protein 2, formerly

Gene ID: 1386, 81647

NCBI Accession: NP_001871

Pathways: MAPK Signaling, RTK Signaling, Thyroid Hormone Synthesis, Activation of Innate immune Response, Chromatin Binding, Myometrial Relaxation and Contraction, Synaptic Membrane, Tube Formation, Toll-Like Receptors Cascades