ATF2 antibody (Activating Transcription Factor 2) (C-Term)

Details for Product anti-ATF2 Antibody No. ABIN184639
Target
Binding Specificity
C-Term
45
32
30
28
27
26
25
23
21
19
18
15
13
12
12
11
11
10
8
8
7
6
5
5
5
5
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
421
281
256
33
26
19
18
13
11
8
7
5
3
2
2
1
1
Host
Goat
391
22
7
3
Clonality
Polyclonal
Conjugate
This ATF2 antibody is un-conjugated
23
17
16
16
15
15
15
15
13
10
8
8
8
8
5
5
4
3
Application
ELISA, Western Blotting (WB)
256
135
121
107
88
77
48
16
12
8
7
6
5
2
2
1
Options
Purpose ATF2
Immunogen Peptide with sequence C-IVMAPSSQSQPSGS, from the C Terminus of the protein sequence according to NP_001871.2.
Blocking Peptide Blocking peptide for this product available: ABIN369231
Sequence IVMAPSSQSQ PSGS
Isotype IgG
Predicted Reactivity Human, Rat, Dog, Cow
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Target
Alternative Name ATF2 (ATF2 Antibody Abstract)
Background ATF2, activating transcription factor 2, HB16, CREB2, TREB7, CRE-BP1, cAMP responsive element binding protein 2, MGC111558, activating transcription factor 2 splice variant ATF2-var2, cAMP responsive element binding protein 2, formerly
Gene ID 1386
NCBI Accession NP_001871
Pathways MAPK Signaling, RTK Signaling, Thyroid Hormone Synthesis, Activation of Innate immune Response, Chromatin Binding, Myometrial Relaxation and Contraction, Synaptic Membrane, Tube Formation, Toll-Like Receptors Cascades
Application Notes

DS WB Results: Approx 75 kDa observed in nuclear lysates of cell lines HeLa, HepG2 and K562. This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Images
Image no. 1 for anti-Activating Transcription Factor 2 (ATF2) (C-Term) antibody (ABIN184639) ABIN184639 staining (0.5µg/ml) of Human Placenta lysate (RIPA buffer, 35µg total prot...