CHFR antibody (Internal Region)

Catalog No. ABIN7223476

This Rabbit Polyclonal antibody specifically detects CHFR in ELISA and WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CHFR antibody (Internal Region) (ABIN7223476)

Target: CHFR (Checkpoint with Forkhead and Ring Finger Domains (CHFR))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHFR antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details anti-CHFR Antibody

Binding Specificity: Internal Region

Purpose: Rabbit Anti-Chfr Polyclonal Antibody

Specificity: Chfr Polyclonal Antibody detects endogenous levels of Chfr protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human Chfr

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:5000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CHFR

Target: CHFR (Checkpoint with Forkhead and Ring Finger Domains (CHFR))

Alternative Name: Chfr

Background: CHFR, RNF196, E3 ubiquitin-protein ligase CHFR, Checkpoint with forkhead and RING finger domains protein, RING finger protein 196CHFR (checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase) encodes an E3 ubiquitin-protein ligase required for the maintenance of the antephase checkpoint that regulates cell cycle entry into mitosis and, therefore, may play a key role in cell cycle progression and tumorigenesis. The encoded protein has an N-terminal forkhead-associated domain, a central RING-finger domain, and a cysteine-rich C-terminal region. Alternatively spliced transcript variants that encode different protein isoforms have been described.

Molecular Weight: 75kD

Gene ID: 55743

UniProt: Q96EP1