ARHGEF4 antibody (N-Term)

Catalog No. ABIN185088

The Goat Polyclonal anti-ARHGEF4 antibody (ABIN185088) specifically detects ARHGEF4 in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for ARHGEF4 antibody (N-Term) (ABIN185088)

Target: ARHGEF4 (rho Guanine Nucleotide Exchange Factor (GEF) 4 (ARHGEF4))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ARHGEF4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-ARHGEF4 Antibody

Binding Specificity: N-Term

Purpose: ARHGEF4

Sequence: PWEEPAGEKP SCS

Specificity: This antibody is expected to recognise both reported isoforms (NP_056135.2, NP_127462.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence PWEEPAGEKPSCS, from the N Terminus of the protein sequence according to NP_056135.2, NP_127462.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 80 kDa band observed in Human Brain lysates and in transfected HEK293 transiently expressing ARHGEF4 (calculated MW of 79.1 kDa according to NP_056135.2 and 76.4 kDa according to 127462.1). Recommended concentration: 2-4 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ARHGEF4

Target: ARHGEF4 (rho Guanine Nucleotide Exchange Factor (GEF) 4 (ARHGEF4))

Alternative Name: ARHGEF4

Background: ARHGEF4, Rho guanine nucleotide exchange factor (GEF) 4, ASEF, GEF4, STM6, KIAA1112, DKFZp434G2016, APC-stimulated guanine nucleotide exchange factor, ASEF1, Rho guanine nucleotide exchange factor 4

Gene ID: 50649

NCBI Accession: NP_056135, NP_127462

Pathways: Neurotrophin Signaling Pathway