Choline Acetyltransferase antibody (C-Term)

Catalog No. ABIN185344

This Goat Polyclonal antibody specifically detects Choline Acetyltransferase in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for Choline Acetyltransferase antibody (C-Term) (ABIN185344)

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Choline Acetyltransferase antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-Choline Acetyltransferase Antibody

Binding Specificity: C-Term

Purpose: Choline acetyltransferase

Sequence: CKEKATRPSQ GHQP

Specificity: This antibody is expected to recognise isoform 1 (NP_066264.3), isoform 2 (NP_065574.3) and isoform 3 (NP_001136405.1). Reported variants represent identical protein (NP_066264.3, NP_066265.3, NP_066266.3, NP_001136406.1, NP_001136401.1).

Cross-Reactivity: Cow, Dog, Human, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence CKEKATRPSQGHQP, from the C Terminus of the protein sequence according to NP_066264.3, NP_065574.3, NP_001136405.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 70 kDa band observed in Human Placenta lysates (calculated MW of 70.5 kDa according to NP_065574 and NP_066264). Recommended concentration: 0.03-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Choline Acetyltransferase

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Alternative Name: CHAT

Background: CHAT, choline acetyltransferase, CMS1A, CMS1A2, acetyl CoA:choline O-acetyltransferase

Gene ID: 1103

NCBI Accession: NP_066264, NP_065574, NP_001136405

Pathways: Skeletal Muscle Fiber Development