PCSK9 antibody (C-Term)

Catalog No. ABIN185371

This Goat Polyclonal antibody specifically detects PCSK9 in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for PCSK9 antibody (C-Term) (ABIN185371)

Target: PCSK9 (Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PCSK9 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-PCSK9 Antibody

Binding Specificity: C-Term

Purpose: PCSK9 (C Terminus)

Sequence: CRSRHLAQAS QELQ

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence CRSRHLAQASQELQ, from the C Terminus of the protein sequence according to NP_777596.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Liver. Recommended concentration: 5-6 μg/mL.

Western Blot: Approx 75 kDa band observed in Human Adipose lysates (calculated MW of 74.3 kDa according to NP_777596.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour. Constructs and lysates were as described in Sun et al Hum Mol Gen

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PCSK9

Target: PCSK9 (Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9))

Alternative Name: PCSK9

Background: PCSK9, FH3, NARC1, HCHOLA3, proprotein convertase subtilisin/kexin type 9, neural apoptosis regulated convertase 1, hypercholesterolemia, autosomal dominant 3, proprotein convertase subtilisin\/kexin type 9, LDLCQ1, NARC-1

Gene ID: 255738

NCBI Accession: NP_777596