HNF4A antibody (N-Term)

Catalog No. ABIN185378

The Goat Polyclonal anti-HNF4A antibody has been validated for WB, ELISA and IHC. It is suitable to detect HNF4A in samples from Human.

Quick Overview for HNF4A antibody (N-Term) (ABIN185378)

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This HNF4A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-HNF4A Antibody

Binding Specificity: N-Term

Purpose: HNF4A

Sequence: RLSKTLVDMD MADY

Specificity: This antibody is expected to recognise the reported isoforms a, b and c (NP_849180.1, NP_000448.3, NP_849181.1 resp.).

Cross-Reactivity: Dog, Human, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence RLSKTLVDMDMADY-C, from the N Terminus of the protein sequence according to NP_849180.1, NP_000448.3, NP_849181.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Small Intestine. Recommended concentration: 2 μg/mL.

Western Blot: Approx 55 kDa and 48 kDa bands observed in lysates of cell line HepG2 (calculated MW of 52.8 kDa according to Human NP_000448.3 , 46.6 kDa according to Human NP_849181.1). Recommended concentration: 0.1-0.5 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for HNF4A

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Alternative Name: HNF4A

Background: HNF4A, TCF, HNF4, NR2A1, TCF14, HNF4a7, HNF4a8, HNF4a9, NR2A21, hepatocyte nuclear factor 4, alpha, HNF4-alpha, transcription factor-14, FLJ39654, HNF4alpha, MODY, MODY1, HNF4alpha10/11/12, hepatic nuclear factor 4 alpha

Gene ID: 3172

NCBI Accession: NP_849180, NP_000448, NP_849181

Pathways: AMPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process