ERK1 antibody (N-Term)

Catalog No. ABIN185399

The Goat Polyclonal anti-ERK1 antibody has been validated for WB, ELISA, IF and FACS. It is suitable to detect ERK1 in samples from Human.

Quick Overview for ERK1 antibody (N-Term) (ABIN185399)

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ERK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-ERK1 Antibody

Binding Specificity: N-Term

Purpose: MAPK3/ERK1

Sequence: GGEPRRTEGV GP

Specificity: No cross-reactivity expected with ERK2

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence GGEPRRTEGVGP-C, from the N Terminus of the protein sequence according to NP_002737.2, NP_001035145.1, NP_001103361.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 40 kDa band observed in Human Cerebellum lysates and in lysates of cell lines A431 and HepG2, and approx 42 kDa obseved in Human Frontal Cortex lysates (calculated MW of 40.1 kDa according to NP_001035145.1 and 43.1 kDa according to NP_0

Peptide ELISA: antibody detection limit dilution 1:4000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and A431 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ERK1

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Alternative Name: MAPK3

Background: MAPK3, ERK1, mitogen-activated protein kinase 3, HGNC:6877, P44ERK1, P44MAPK, PRKM3, protein kinase, mitogen-activated 3 (MAP kinase 3 p44), HS44KDAP, HUMKER1A, MGC20180, OTTHUMP00000174538, extracellular signal-regulated kinase 1, extracellular signal-re

Gene ID: 5595

NCBI Accession: NP_002737, NP_001035145, NP_001103361

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins