ENPEP antibody (Internal Region)

Catalog No. ABIN185416

This Goat Polyclonal antibody specifically detects ENPEP in WB, IHC and ELISA. It exhibits reactivity toward Human and Rat.

Quick Overview for ENPEP antibody (Internal Region) (ABIN185416)

Target: ENPEP (Glutamyl Aminopeptidase (Aminopeptidase A) (ENPEP))

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This ENPEP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-ENPEP Antibody

Binding Specificity: Internal Region

Purpose: Aminopeptidase A

Sequence: EQYQKTSLAQ EKEK

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EQYQKTSLAQEKEK, from the internal region of the protein sequence according to NP_001968.3.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Kidney shows staining of basement membrane and the mesangium in the glomerulus. Recommended concentration: 3-5 μg/mL.

Western Blot: Approx 170 kDa band observed in Human Brain and Testis lysates (calculated MW of 109 kDa according to NP_001968.2). Primary incubation was for 1 hour.The observed molecular weight corresponds to earlier findings in literature with different

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ENPEP

Target: ENPEP (Glutamyl Aminopeptidase (Aminopeptidase A) (ENPEP))

Alternative Name: ENPEP

Background: Aminopeptidase A, ENPEP, glutamyl aminopeptidase (aminopeptidase A) , HGNC:3355, APA, gp160, CD249, glutamyl aminopeptidase

Gene ID: 2028, 13809

NCBI Accession: NP_001968

Pathways: Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones