VPS11 antibody (Internal Region)

Catalog No. ABIN185427

The Goat Polyclonal anti-VPS11 antibody (ABIN185427) specifically detects VPS11 in ELISA and IHC. The antibody is reactive with Human samples.

Quick Overview for VPS11 antibody (Internal Region) (ABIN185427)

Target: VPS11 (Vacuolar Protein Sorting 11 Homolog (VPS11))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This VPS11 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-VPS11 Antibody

Binding Specificity: Internal Region

Purpose: VPS11

Sequence: HALQEKDTQT KLE

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-HALQEKDTQTKLE, from the internal region of the protein sequence according to NP_068375.3.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Kidney shows brushborder staning in PCT cells and Human Pancreas shows cytoplasm staining of beta cells in islets of Langerhans. Recommended concentration, 3-6 μg/mL.

Western Blot: Preliminary experiments gave an approx 75 kDa band in human duodenum, colon and placenta lysates after 0.3 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the c

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for VPS11

Target: VPS11 (Vacuolar Protein Sorting 11 Homolog (VPS11))

Alternative Name: VPS11

Background: VPS11, vacuolar protein sorting 11 (yeast) , HGNC:14583, END1, PEP5, RNF108, hVPS11 , vacuolar protein sorting 11 (yeast homolog), vacuolar protein sorting protein 11, vacuolar protein sorting 11 homolog (S. cerevisiae)

Gene ID: 55823, 71732

NCBI Accession: NP_068375

Pathways: SARS-CoV-2 Protein Interactome