PCK1 antibody (Internal Region)

Catalog No. ABIN185482

This Goat Polyclonal antibody specifically detects PCK1 in ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for PCK1 antibody (Internal Region) (ABIN185482)

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PCK1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-PCK1 Antibody

Binding Specificity: Internal Region

Purpose: PCK1 / PEPCKC (internal)

Sequence: HVNWFRKDKE GK

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-HVNWFRKDKEGK, from the internal region of the protein sequence according to NP_002582.3.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Cerebral Cortex, Kidney and Liver. Recommended concentration: 2 μg/mL.

Western Blot: Preliminary experiments gave bands at approx 70 kDa, 45 kDa and 28 kDa in Human Adipose, Kidney and Liver lysates after 0.3 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the additional

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PCK1

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Alternative Name: PCK1

Background: PCK1, phosphoenolpyruvate carboxykinase 1 (soluble), HGNC:8724, MGC22652, PEPCK1, PEPCKC, PEP carboxykinase, cytosolic phosphoenolpyruvate carboxykinase 1, phosphoenolpyruvate carboxylase, phosphopyruvate carboxylase, PEPCK-C

Gene ID: 5105

NCBI Accession: NP_002582

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis