Choline Acetyltransferase antibody (C-Term)

Catalog No. ABIN185635

This Goat Polyclonal antibody specifically detects Choline Acetyltransferase in WB, IHC and ELISA. It exhibits reactivity toward Mouse and Rat.

Quick Overview for Choline Acetyltransferase antibody (C-Term) (ABIN185635)

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Reactivity: Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This Choline Acetyltransferase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-Choline Acetyltransferase Antibody

Binding Specificity: C-Term

Purpose: Chat (mouse)

Sequence: KERARGPTKP SNLD

Specificity: This antibody is NOT expected to cross-react to Human CHAT.

Cross-Reactivity: Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KERARGPTKPSNLD, from the C Terminus of the protein sequence according to NP_034021.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Frozen sections of Mouse Forebrain shows staining of neural cell bodies. Optimal dilution: 1:2000.

Western Blot: Approx 75 kDa band observed in Mouse and Rat Brain lysates (calculated MW of 72.3 kDa according to Mouse NP_034021.1 and Rat NP_001164064.1). Recommended concentration:0.3-1 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Choline Acetyltransferase

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Alternative Name: Chat

Background: Chat, choline acetyltransferase , B230380D24Rik

Gene ID: 12647, 432359

NCBI Accession: NP_034021

Pathways: Skeletal Muscle Fiber Development