CPT1A antibody (Internal Region)

Catalog No. ABIN185710

This Goat Polyclonal antibody specifically detects CPT1A in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for CPT1A antibody (Internal Region) (ABIN185710)

Target: CPT1A (Carnitine Palmitoyltransferase 1A (Liver) (CPT1A))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CPT1A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-CPT1A Antibody

Binding Specificity: Internal Region

Purpose: CPT1A

Sequence: DPAQTVEQRL KLFK

Specificity: This antibody is expected to recognize both reported isoforms (NP_001867.2, NP_001027017.1) but is not expected to recognise CPT1B.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DPAQTVEQRLKLFK, from the internal region of the protein sequence according to NP_001867.2, NP_001027017.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Liver. Recommended concentration: 5-7 μg/mL.

Western Blot: Approx 80-85 kDa band observed in lysates of cell line MCF7 (calculated MW of 86.2 kDa according to NP_001027017.1). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CPT1A

Target: CPT1A (Carnitine Palmitoyltransferase 1A (Liver) (CPT1A))

Alternative Name: CPT1A

Background: CPT1A, carnitine palmitoyltransferase 1A (liver) , CPT1, CPT1-L, L-CPT1 , carnitine palmitoyltransferase 1A, carnitine palmitoyltransferase I, carnitine palmitoyltransferase I, liver

Gene ID: 1374

NCBI Accession: NP_001867, NP_001027017

Pathways: AMPK Signaling, Regulation of Lipid Metabolism by PPARalpha, Feeding Behaviour, Monocarboxylic Acid Catabolic Process