CDK7 antibody (AA 12-295)

Catalog No. ABIN1858349

The Rabbit Polyclonal anti-CDK7 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect CDK7 in samples from Mouse.

Quick Overview for CDK7 antibody (AA 12-295) (ABIN1858349)

Target: CDK7 (Cyclin-Dependent Kinase 7 (CDK7))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-CDK7 Antibody

Binding Specificity: AA 12-295

Purpose: Polyclonal Antibody to Cyclin Dependent Kinase 7 (CDK7)

Specificity: The antibody is a rabbit polyclonal antibody raised against CDK7. It has been selected for its ability to recognize CDK7 in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: CDK7 (Tyr12-Phe295)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CDK7

Target: CDK7 (Cyclin-Dependent Kinase 7 (CDK7))

Alternative Name: CDK7

Background: CAK1, CDKN7, STK1, MO15, 39 kDa protein kinase, Serine/threonine-protein kinase 1, Cell Division Protein Kinase 7, TFIIH basal transcription factor complex kinase subunit

Pathways: Cell Division Cycle, DNA Damage Repair, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, M Phase