HMGN1 antibody (AA 14-100)

Catalog No. ABIN1859181

This anti-HMGN1 antibody is a Rabbit Polyclonal antibody detecting HMGN1 in WB, ELISA, IHC (p), ICC and IHC (fro). Suitable for Human.

Quick Overview for HMGN1 antibody (AA 14-100) (ABIN1859181)

Target: HMGN1 (High Mobility Group Nucleosome Binding Domain 1 (HMGN1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMGN1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-HMGN1 Antibody

Binding Specificity: AA 14-100

Purpose: Polyclonal Antibody to High Mobility Group Nucleosome Binding Domain Protein 1 (HMGN1)

Specificity: The antibody is a rabbit polyclonal antibody raised against HMGN1. It has been selected for its ability to recognize HMGN1 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant High Mobility Group Nucleosome Binding Domain Protein 1 (HMGN1) corresdonding to Lys14~Asp100 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.36 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for HMGN1

Target: HMGN1 (High Mobility Group Nucleosome Binding Domain 1 (HMGN1))

Alternative Name: HMGN1

Background: IPO38, HMG14, Non-histone chromosomal protein HMG-14

Pathways: Chromatin Binding