NEK2 antibody (AA 148-397)
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- Target See all NEK2 Antibodies
- NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))
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Binding Specificity
- AA 148-397
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Reactivity
- Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This NEK2 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Purpose
- Polyclonal Antibody to Never In Mitosis Gene A Related Kinase 2 (NEK2)
- Specificity
- The antibody is a rabbit polyclonal antibody raised against NEK2. It has been selected for its ability to recognize NEK2 in immunohistochemical staining and western blotting.
- Cross-Reactivity
- Human, Rat
- Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- Immunogen
- Recombinant Never In Mitosis Gene A Related Kinase 2 (NEK2) corresdonding to Phe148~Glu397 (Accession # O35942)
- Isotype
- IgG
- Top Product
- Discover our top product NEK2 Primary Antibody
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- Application Notes
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Western blotting: 0.5-3 μg/mL
Immunohistochemistry: 5-30 μg/mL
Immunocytochemistry: 5-30 μg/mL
Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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- Target
- NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))
- Alternative Name
- Never In Mitosis Gene A Related Kinase 2 (NEK2 Products)
- Synonyms
- AA617254 antibody, C77054 antibody, HsPK21 antibody, NEK2A antibody, NLK1 antibody, ATNEK2 antibody, NIMA-RELATED KINASE2 antibody, NIMA-related kinase 2 antibody, T9J14.24 antibody, T9J14_24 antibody, NIMA related kinase 2 antibody, NIMA (never in mitosis gene a)-related expressed kinase 2 antibody, NIMA-related kinase 2 antibody, NEK2 antibody, Nek2 antibody
- Background
- HsPK 21, HsPK21, NEK2A, NLK1, NimA-like protein kinase 1, Serine/threonine-protein kinase Nek2
- Pathways
- M Phase
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