PKC eta antibody (AA 355-614)
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- Target See all PKC eta (PRKCH) Antibodies
- PKC eta (PRKCH) (Protein Kinase C, eta (PRKCH))
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Binding Specificity
- AA 355-614
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PKC eta antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Purpose
- Polyclonal Antibody to Protein Kinase C Eta (PKCh)
- Specificity
- The antibody is a rabbit polyclonal antibody raised against PKCh. It has been selected for its ability to recognize PKCh in immunohistochemical staining and western blotting.
- Cross-Reactivity
- Mouse, Rat
- Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- Immunogen
- Recombinant Protein Kinase C Eta (PKCh) corresdonding to Phe355~Phe614 (Accession # P24723)
- Isotype
- IgG
- Top Product
- Discover our top product PRKCH Primary Antibody
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- Application Notes
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Western blotting: 1-5 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
- Preservative
- ProClin
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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- Target
- PKC eta (PRKCH) (Protein Kinase C, eta (PRKCH))
- Alternative Name
- Protein Kinase C Eta (PRKCH Products)
- Synonyms
- PKC-L antibody, PKCL antibody, PRKCL antibody, nPKC-eta antibody, Pkch antibody, pkc-eta antibody, PRKCH antibody, prkch antibody, si:ch211-212g7.5 antibody, zgc:172124 antibody, protein kinase C eta antibody, protein kinase C, eta antibody, protein kinase C eta L homeolog antibody, protein kinase C, eta, b antibody, protein kinase C, eta, a antibody, protein kinase C epsilon antibody, PRKCH antibody, Prkch antibody, prkch.L antibody, prkchb antibody, prkcha antibody, prkch antibody, PRKCE antibody
- Background
- PRKCH, PRKC-H, PKC-L, PRKCL, NPKC-Eta
- Pathways
- Myometrial Relaxation and Contraction, Thromboxane A2 Receptor Signaling
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