Glutaminase antibody (AA 31-253)

Catalog No. ABIN1868178

This anti-Glutaminase antibody is a Rabbit Polyclonal antibody detecting Glutaminase in WB and IHC. Suitable for Mouse.

Quick Overview for Glutaminase antibody (AA 31-253) (ABIN1868178)

Target: Glutaminase (GLS)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glutaminase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Glutaminase Antibody

Binding Specificity: AA 31-253

Purpose: Polyclonal Antibody to Glutaminase (GLS)

Specificity: The antibody is a rabbit polyclonal antibody raised against GLS. It has been selected for its ability to recognize GLS in immunohistochemical staining and western blotting.

Cross-Reactivity: Human

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Glutaminase (GLS) corresdonding to Leu31~Asp253 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Glutaminase

Target: Glutaminase (GLS)

Alternative Name: Glutaminase

Background: GLS1, Glutaminase kidney isoform, mitochondrial, K-glutaminase, L-glutamine amidohydrolase

Pathways: Feeding Behaviour, Dicarboxylic Acid Transport, Warburg Effect