PPARA antibody (AA 280-433)

Catalog No. ABIN1869903

Product Details anti-PPARA Antibody

Target: PPARA (Peroxisome Proliferator-Activated Receptor alpha (PPARA))

Binding Specificity: AA 280-433

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPARA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Peroxisome Proliferator Activated Receptor Alpha (PPARa)

Specificity: The antibody is a rabbit polyclonal antibody raised against PPARa. It has been selected for its ability to recognize PPARa in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Peroxisome Proliferator Activated Receptor Alpha (PPARa)

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

Immunohistochemistry: 5-20 μg/mL

Immunocytochemistry: 5-20 μg/mL

Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for PPARA

Target: PPARA (Peroxisome Proliferator-Activated Receptor alpha (PPARA))

Alternative Name: Peroxisome Proliferator Activated Receptor Alpha (PPARA Products)

Synonyms: NR1C1 antibody, PPAR antibody, PPARalpha antibody, hPPAR antibody, LOC100136415 antibody, PPARALPHA antibody, nr1c1 antibody, xPPARalpha antibody, xppara antibody, 4933429D07Rik antibody, AW742785 antibody, Nr1c1 antibody, PPAR-alpha antibody, Ppar antibody, peroxisome proliferator activated receptor alpha antibody, peroxisome proliferator-activated receptor alpha antibody, peroxisome proliferator activated receptor alpha L homeolog antibody, PPARA antibody, LOC100136415 antibody, ppara.L antibody, Ppara antibody, ppara antibody

Background: NR1C1, PPAR-A, HPPAR, Nuclear Receptor Subfamily 1 Group C Member 1

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Regulation of Carbohydrate Metabolic Process, Hepatitis C