GZMB antibody

Catalog No. ABIN187356

The Mouse Monoclonal anti-GZMB antibody (Clone B18-1) (ABIN187356) specifically detects GZMB in WB, FACS, IF, IHC (p) and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for GZMB antibody (ABIN187356)

Target: GZMB (Granzyme B (GZMB))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GZMB antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: B18-1

Product Details anti-GZMB Antibody

Specificity: Recognizes human granzyme B. Species Reactivity: Human. Others not tested. Positive Control: Human CD8+ T cells.

Purity: 95 % as determined by SDS-PAGE.

Immunogen: Recombinant human granzyme B (aa 7-227)

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry Immunocytochemistry Immunoprecipitation Immunohistochemistry: (frozen sections and paraffin sections) Western blot: Suggested dilution of 1 µg/mL The optimal dilution for a specific application should be determined by the researcher. Figure A: Detection of recombinant granzyme B. Granzyme B migrates as a 27 kDa species. Method: Recombinant granzyme B was resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the monoclonal anti-granzyme B antibody B18.1 at 1 µg/mL. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG and a chemiluminescence detection system. Figure B: Detection of granzyme B in a human CD8+ T cell clone. Method: Human T cells were plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20 °C for 5 min., then in acetone at -20 °C for 30 seconds. After 3 washes in PBS, 0.1% BSA, slides were incubated for 1 hour at room temperature (RT) with 20 µg/mL of clone B18.4 antibody in PBS, 0.1% BSA. After rinsing in PBS, a FITC-conjugated anti-mouse IgG was added for 30 min., slides washed again in PBS and visualized using a fluorescence microscope.

Restrictions: For Research Use only

Handling

Buffer: 100 µg of liquid purified monoclonal antibody at 1 mg/mL in PBS with 0.02% sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for GZMB

Target: GZMB (Granzyme B (GZMB))

Background: Granzyme B is a 27 kDa serine protease stored in granules of activated cytotoxic T cells and NK cells. Upon target cell contact, granzyme B is directionally exocytosed and, assisted by perforin, enters the target cell. In the target, granzyme B processes and activates various pro-caspases, inducing apoptosis in the target cell.

Pathways: Apoptosis, Caspase Cascade in Apoptosis