Caspase 9 antibody (Ser196)

Catalog No. ABIN1881137

The Rabbit Polyclonal anti-Caspase 9 antibody has been validated for WB, FACS and IHC (p). It is suitable to detect Caspase 9 in samples from Human. There are 5+ publications available.

Quick Overview for Caspase 9 antibody (Ser196) (ABIN1881137)

Target: Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 9 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB6899

Product Details anti-Caspase 9 Antibody

Binding Specificity: Ser196

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This CASP9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S196 of human CASP9.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:10~50. FC: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-Caspase 9 antibody (ABIN1881137)

Martin, Allan, Lickrish, Sampson, Morrice, Clarke: "Protein kinase A regulates caspase-9 activation by Apaf-1 downstream of cytochrome c." in: The Journal of biological chemistry, Vol. 280, Issue 15, pp. 15449-55, (2005) (PubMed).

Raina, Pandey, Ahmad, Bharti, Ren, Kharbanda, Weichselbaum, Kufe: "c-Abl tyrosine kinase regulates caspase-9 autocleavage in the apoptotic response to DNA damage." in: The Journal of biological chemistry, Vol. 280, Issue 12, pp. 11147-51, (2005) (PubMed).

Cornelis, Bruynooghe, Van Loo, Saelens, Vandenabeele, Beyaert: "Apoptosis of hematopoietic cells induced by growth factor withdrawal is associated with caspase-9 mediated cleavage of Raf-1." in: Oncogene, Vol. 24, Issue 9, pp. 1552-62, (2005) (PubMed).

Mohammad, Wang, Aboukameel, Chen, Wu, Chen, Al-Katib: "Preclinical studies of a nonpeptidic small-molecule inhibitor of Bcl-2 and Bcl-X(L) [(-)-gossypol] against diffuse large cell lymphoma." in: Molecular cancer therapeutics, Vol. 4, Issue 1, pp. 13-21, (2005) (PubMed).

Tacconi, Perri, Balestrieri, Grelli, Bernardini, Annichiarico, Mastino, Caltagirone, Macchi: "Increased caspase activation in peripheral blood mononuclear cells of patients with Alzheimer's disease." in: Experimental neurology, Vol. 190, Issue 1, pp. 254-62, (2004) (PubMed).

Target Details for Caspase 9

Target: Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))

Alternative Name: CASP9

Background: Caspase 9 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1, this step is thought to be one of the earliest in the caspase activation cascade.

Molecular Weight: 46281

NCBI Accession: NP_001220, NP_001264983, NP_127463

UniProt: P55211

Pathways: MAPK Signaling, RTK Signaling, Apoptosis, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Endopeptidase Activity