HIST2H3A antibody (C-Term)

Catalog No. ABIN1881419

Product Details anti-HIST2H3A Antibody

Target: HIST2H3A (Histone Cluster 2, H3a (HIST2H3A))

Binding Specificity: AA 108-136, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIST2H3A antibody is un-conjugated

Application: Western Blotting (WB)

Predicted Reactivity: B, M, Rat, C, Zf, X, E, D, Pig, Rb

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This HIST2H3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 108-136 amino acids from the C-terminal region of human HIST2H3A.

Clone: RB41556

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-HIST2H3A antibody (ABIN1881419)

Neumann, Hancock, Buning, Routh, Chapman, Somers, Owen-Hughes, van Noort, Rhodes, Chin: "A method for genetically installing site-specific acetylation in recombinant histones defines the effects of H3 K56 acetylation." in: Molecular cell, Vol. 36, Issue 1, pp. 153-63, (2009) (PubMed).

Hurd, Bannister, Halls, Dawson, Vermeulen, Olsen, Ismail, Somers, Mann, Owen-Hughes, Gout, Kouzarides: "Phosphorylation of histone H3 Thr-45 is linked to apoptosis." in: The Journal of biological chemistry, Vol. 284, Issue 24, pp. 16575-83, (2009) (PubMed).

Yuan, Pu, Zhang, Lou: "Histone H3-K56 acetylation is important for genomic stability in mammals." in: Cell cycle (Georgetown, Tex.), Vol. 8, Issue 11, pp. 1747-53, (2009) (PubMed).

Chang, Zhang, Beezhold, Bhatia, Zhao, Chen, Castranova, Shi, Chen: "Sustained JNK1 activation is associated with altered histone H3 methylations in human liver cancer." in: Journal of hepatology, Vol. 50, Issue 2, pp. 323-33, (2009) (PubMed).

Kobza, Sarath, Zempleni: "Prokaryotic BirA ligase biotinylates K4, K9, K18 and K23 in histone H3." in: BMB reports, Vol. 41, Issue 4, pp. 310-5, (2008) (PubMed).

Target Details for HIST2H3A

Target: HIST2H3A (Histone Cluster 2, H3a (HIST2H3A))

Alternative Name: HIST2H3A (HIST2H3A Products)

Synonyms: H3/n antibody, H3/o antibody, histone cluster 2 H3 family member a antibody, HIST2H3A antibody

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated, this record represents the telomeric copy. [provided by RefSeq].

Molecular Weight: 15388

NCBI Accession: NP_001005464, NP_001116847, NP_066403

UniProt: Q71DI3