CDK4 antibody (N-Term)

Catalog No. ABIN7217934

This Rabbit Polyclonal antibody specifically detects CDK4 in WB, ELISA, IHC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CDK4 antibody (N-Term) (ABIN7217934)

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CDK4 Antibody

Binding Specificity: N-Term

Purpose: Rabbit Anti-Cdk4 Polyclonal Antibody

Specificity: Cdk4 Polyclonal Antibody detects endogenous levels of Cdk4 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human Cdk4

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IF 1:50-200,WB 1:500-1:2000,IHC: 1:100-300,ELISA 1:20000,Not yet tested in other applications,

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CDK4

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Alternative Name: Cdk4

Background: CDK4, Cyclin-dependent kinase 4, Cell division protein kinase 4, PSK-J3Cyclin-dependent kinase 4 encoded by CDK4 is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16 (INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16 (INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.

Molecular Weight: 35kD

Gene ID: 1019

UniProt: P11802

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size